Professor Guoji Guo is the deputy director of the Center for Stem Cell and Regenerative Medicine at Zhejiang University School of Medicine. He is also the deputy chair of Stem Cell Society at Zhejiang University. He obtained his Ph.D at National University of Singapore in 2005, and then moved to Harvard Medical School for postdoc training. In 2014, he was recruited and directly tenured at Zhejiang University. In 2015, he was selected for the 1000 Youth Talent Plan of China. In 2017, he was awarded the National Science Fund for Excellent Young Scholars. Professor Guoji Guo is interested in developing new single cell analysis technologies and applying these technologies to investigate stem cell biology. He is also looking for novel methods that enable stem cell expansion in vitro.
Mapping the Mouse Cell Atlas by Microwell-seq
Guoji GuoCenter for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China
Single cell RNA-seq (scRNA-seq) technologies are poised to reshape the current cell type classification system. However, a transcriptome-based single cell atlas has not been achieved for complex mammalian systems. Here, we developed Microwell-seq, a high-throughput and low-cost scRNA-seq platform using simple, inexpensive devices. Using Microwell-seq, we analyzed more than 400,000 single cells covering all of the major mouse organs and constructed a basic scheme for a Mouse Cell Atlas (MCA). We reveal a single cell hierarchy for many tissues that have not been well characterized previously. We built a web-based “Single Cell MCA Analysis” pipeline that accurately defines cell types based on single cell digital expression. Our study demonstrates the wide applicability of the Microwell-seq technology and MCA resource.
Han X, Wang R, Zhou Y, Fei L, Sun H, Lai S, Saadatpour A, Zhou Z, Chen H, Ye F, Huang D, Xu Y, Huang W, Jiang M, Jiang X, Mao J, Chen Y, Lu C, Xie J, Fang Q, Wang Y, Yue R, Li T, Huang H, Orkin SH, Yuan GC, Chen M, Guo G. Mapping the Mouse Cell Atlas by Microwell-seq. Cell. 2018 Feb 22;172(5):1091-1107..
Han X, Yu H, Huang D, Xu Y, Saadatpour A, Li X, Wang L, Yu J, Pinello L, Lai S, Jiang M, Tian X, Zhang F, Cen Y, Fujiwara Y, Zhu W, Zhou B, Zhou T, Ouyang H, Wang J, Yuan GC, Duan S, Orkin SH, Guo G. A molecular roadmap for induced multi-lineage trans-differentiation of fibroblasts by chemical combinations. Cell Res. 2017 Mar;27(3):386-401.
Guo G, Luc S, Marco E, Lin TW, Peng C, Kerenyi MA, Beyaz S, Kim W, Xu J, Das PP, Neff T, Zou K, Yuan GC, Orkin SH. Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire. Cell Stem Cell. 2013 Oct 3;13(4):492-505.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P. Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85.