Speakers at ICG-13

Speakers at ICG-13

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Biography

Yanli Wang is also a HHMI International Research Scholar. Prof. Wang has completed her PhD from University of Science and Technology of China and postdoctoral studies from Memorial Sloan-Kettering Cancer Center. Prof. Wang's group interests in understanding how small regulatory RNA or DNA mediates prokaryotic defense against invasion by foreign nucleic acids. The main focus of her work is to systematically demonstrate the mechanisms of the CRISPR-Cas system, and Ago protein-mediated DNA interference.


Abstract

CRISPR-Cas Mediated Cleavage of Invading Nucleic Acids

Liang Liu1, Xueyan Li1, 2, Jiuyu Wang1, Min Wang1, Jiazhi Li1, 2,Hongtu Zhao1, 2,

 Maolu Yin1, 2, Gang Sheng1 and Yanli Wang1, 2

1Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

2University of Chinese Academy of Sciences, Beijing 100049, China

Bacteria and archaea are protected against invading nucleic acids from phages and plasmids because of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR associated proteins) systems, which are RNA-guided prokaryotic adaptive immune system. CRISPR-Cas systems are found in nearly half of all bacteria studied so far, as well as in the majority of archaea. However, throughout evolution, this host defense system has not resulted in the eradication of phages, suggesting that phages have evolved counter strategies to thrive within bacteria despite these mechanisms. Thus, both bacterial CRISPR system and phage anti-CRISPR system are part of a continuing evolutionary battle between bacterial host and their bacteriophage invaders. We provided significant insights into the molecular mechanism for how CRISPR-Cas systems defend against the invading nucleic acids from phages and how phages counteract the CRISPR-Cas systems by anti-CRIPR proteins.   

 

References

1.           Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y. (2017) The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. Cell. 170: 714-726

2.           Liang Liu, Xueyan Li, Jiuyu Wang,Maolu Yin, Peng Chen, Min Wang, Jiazhi Li, Gang Sheng and Yanli Wang*. (2017) Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. Cell. 168:121-134.

3.           Wang, J., Ma, J., Cheng, Z., Meng, X., You, L., Wang, M., Zhang, X., and Wang, Y. (2016) A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. Cell Res 26, 1165-1168

4.           Wang, J., Li, J., Zhao, H., Sheng, G., Wang, M., Yin, M., and Wang, Y. (2015) Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell 163, 840-853

Zhao, H., Sheng, G., Wang, J., Wang, M., Bunkoczi, G., Gong, W., Wei, Z., and Wang, Y. (2014) Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 515, 147-150
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